A COMPREHENSIVE SCHEME FOR THE SYSTEMATIC INVESTIGATION OF HEMOLYTIC-ANEMIA

A comprehensive scheme for the systematic investigation of hemolytic a nemia is proposed. In addition to routinely available tests for the di agnosis of hemolytic anemia, investigation of hemoglobin, analysis of red blood cell membrane proteins, and a complete panel of red blood ce ll enzyme measurements were included. The adoption of red blood cell e nzyme measurements to routine use was facilitated by the automation of these procedures. The resolving power of sodium dodecyl sulfate-polya crylamide gel electrophoresis(SDS-PAGE) procedure provided a tool for the identification of abnormalities in the red blood cell membrane pro teins. Such a comprehensive scheme was instrumental in systematically identifying and confirming 48 cases of hemolytic anemia which otherwis e would have escaped detection during the period October 1990 to Septe mber 1993 in our institution (Fukuoka University Hospital).