EFFECT OF AMMONIUM-CHLORIDE ON MULTIPLICATION OF RINDERPEST VIRUS IN VERO CELLS

Amonium chloride, a lysosomotropic weak base, inhibited replication of rinderpest virus in Vero cells. The inhibition of replication was dos e-dependent and the minimum effective dose of ammonium chloride was de termined as 5 - 10 mmol/l. The fusion efficiency and the yield of both cell-free and cell-associated virus were reduced in the presence of t he inhibitor. Western blot analysis of rinderpest virus-infected Vero cells revealed that synthesis of two virus-induced polypeptides were a ffected by the presence of ammonium chloride.