TITRATION OF NUMBERS OF HUMAN-DERIVED MYCOBACTERIUM-LEPRAE REQUIRED TO PROGRESSIVELY OXIDIZE C-14 PALMITIC ACID AND RELEASE CO-14(2)

Authors
SHANNON EJ FROMMEL D GUEBREXABIER M HAILEMARIAM HS
Citation
Ej. Shannon et al., TITRATION OF NUMBERS OF HUMAN-DERIVED MYCOBACTERIUM-LEPRAE REQUIRED TO PROGRESSIVELY OXIDIZE C-14 PALMITIC ACID AND RELEASE CO-14(2), Leprosy review, 65(2), 1994, pp. 100-105
Citations number
8
Categorie Soggetti
Tropical Medicine",Pathology,"Dermatology & Venereal Diseases
Journal title
Leprosy review → ACNP
ISSN journal
03057518
Volume
65
Issue
2
Year of publication
1994
Pages
100 - 105
Database
ISI
SICI code
0305-7518(1994)65:2<100:TONOHM>2.0.ZU;2-K
Abstract
Mycobacterium leprae was isolated from skin-punch biopsies of 2 untrea ted lepromatous leprosy patients. The bacteria were enumerated, dilute d 10-fold and cultured in Middlebrook 7H9 medium supplemented with alb umin, dextrose, catalase and C-14-palmitic acid. The cultures were inc ubated at 33-degrees-C in a modified Buddemeyer radiorespiratory detec tion vessel. Those cultures containing at least 10(7) mycobacteria dem onstrated a progressive evolution of (CO2)-C-14.