EXTRACTIVES FROM THE BARK OF ABIES-SACHALINENSIS .1. THE EFFICIENT EXTRACTION OF CIS-ABIENOL USING SUPERCRITICAL CARBON-DIOXIDE

The dichloromethane extractives from the bark of Abies sachalinensis M ast. were studied. Three diterpenoids, cis-abienol (I), 13-epi-manool (II), and dehydroabietic acid (III), and two triterpenoids, beta-sitos terol (IV) and beta-sitostenone (V), were isolated from its bark. A ra pid and quantitative analysis method for cis-abienol was studied by us ing the HPLC system to develop an efficient extraction method. Efficie nt extractions of cis-abienol from the bark have been studied by means of organic solvents and supercritical carbon dioxide. Consequently, i t was revealed that supercritical carbon extraction under 100 kgf/cm2 at 40-degrees-C was the most efficient method for extracting cis-abien ol from the bark. Under the above conditions, the content of cis-abien ol was 13.60% in extractives (0.023% on an oven-dried bark basis).