Helium isotopes have been measured in fluids trapped in minerals from
three mid-ocean ridge hydrothermal systems at bare-rock sites and at t
he sediment-covered site at Middle Valley, northern Juan de Fuca Ridge
. Fluid-inclusion He-3/He-4 ratios from the bare-rock sites are indist
inguishable from those of the vent fluids and are close to the local b
asalt values. Values for deltaS-34 of the host sulfide are consistent
with variable contributions of seawater sulfate S to the basalt-derive
d S in the hydrothermal fluids. This does not affect inclusion-hosted
He-3/He-4 because of the high He concentration of the hydrothermal flu
ids relative to seawater. Fluid-inclusion He-3/He-4 ratios from Middle
Valley are a mixture of basalt-derived He and radiogenic He acquired
from pore fluids in the overlying Pleistocene turbidite sediments afte
r seawater-basalt interaction. The radiogenic He accompanies isotopica
lly heavy S, demonstrating that He isotopes may be used to distinguish
pore fluids from seawater in hydrothermal systems.