DIFFERENT EVENTS INVOLVED IN THE INDUCTION OF MACROPHAGE TUMOR-NECROSIS-FACTOR BY CANDIDA-ALBICANS AND LIPOPOLYSACCHARIDE

Using an in vitro experimental model, we have recently demonstrated th at Candida albicans in its hyphal form (H-Candida), similarly to lipop olysaccharide (LPS), enhances tumor necrosis factor (TNF) secretory re sponse in the cloned macrophage (M phi) population ANA-1. Here we show that H-Candida and LPS each differ in their requirements for intact p rotein kinase functions, susceptibility to 0.4-mu m micropore-size mem branes, and sensitivity to polymyxin B. These results, together with t he synergistic effect occurring between H-Candida and LPS in inducing TNF response, indicate the existence of different receptor(s) and/or s ignal-transduction pathway(s) through which the two stimuli act. (C) 1 994 Academic Press, Inc.