The in vitro technique for estimating relative digestion rates in rumi
nants on various forages has created conflicting results in a number o
f investigations. Some studies show both inter- and intraspecific vari
ation in the ability of inocula to digest the same substrate, while th
e results of other studies do not verify this potential source of erro
r. This study was designed to compare variation in in vitro dry matter
digestibility (IVDMD) using inocula from 8 different moose. Further,
samples were taken from 3 different parts of the rumen, cranial sac, v
entral sac, and ventral blind sac, in order to look for potential vari
ation in inoculum quality within the rumen. The moose were collected o
n 3 consecutive days in October 1990. Current year growth of Scots pin
e (Pinus sylvestris L.) collected immediately before the experiment wa
s used as substrate. The results showed considerable differences betwe
en inocula from different animals in digestion of the substrate. Sex o
r age of inoculum donor did not account for the variability, and site
of origin from within the rumen had a significant impact on IVDMD only
in 1 animal. A botanical analysis of the rumen contents from the moos
e showed considerably variation in the proportion of pine and dwarf sh
rubs (Ericaceae), the main food types consumed. These feeding differen
ces were not reflected in the IVDMD results.