GENETIC-STRUCTURE OF PYRENOPHORA-TERES POPULATIONS DETERMINED WITH RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS

The genetic structure of Pyrenophora teres, an ascomycete fungus that causes net blotch of barley, was examined using random amplified polym orphic DNA (RAPD) markers. Twenty-seven random oligonucleotide primers were screened against DNA from 16 isolates of P. teres of diverse geo graphic origin. Five primers gave scorable, reproducible DNA products (bands) suitable for population genetic studies. Genetic analyses of b ands produced by two of the primers revealed single locus segregation in three of four crosses, indicating that these RAPDs can be interpret ed as alleles at genetic loci. Allele frequencies were determined for 10 putative RAPD loci from five primers in 22-35 isolates of P. teres sampled from each of five geographically separated populations in Cana da, Germany, and the U.S.A. Eight RAPD loci were polymorphic in at lea st one population and two loci were monomorphic in all five population s. Variation in allele frequencies (allelic diversity) among the five populations was partitioned into within- and among-population componen ts using Nei's G(ST). A G(ST) value of 0.46 was obtained among all pop ulations indicating that approximately 46% of the total genetic variab ility detected was due to differentation among populations compared wi th 54% within populations. A G(ST) value of 0.33 was obtained among th e North American populations only. From five to nine multilocus genoty pes were found in each population. Nine genotypes occurred exclusively in the German population and four exclusively in the New York populat ion. The other populations had one or two unique genotypes. Gametic di sequilibrium values (nonrandom associations of RAPD loci) were calcula ted among all pairs of polymorphic loci within each population. Eleven of 49 values were significantly different from 0 (P < 0.05); 8 of the 11 significant gametic disequilibrium values occurred in the New York population. Highly significant gametic disequilibrium was detected be tween the same two RAPD loci in three different populations, suggestin g that these loci are genetically linked. Two different multilocus ana lyses revealed that the genetic structures of the Alberta, North Dakot a, and German populations but not the New York population were consist ent with random sexual reproduction occurring in these populations.