A COMPETITIVE AVIDIN-BIOTIN ECL DOT-BLOT ASSAY FOR EPITOPE MAPPING OFMONOCLONAL-ANTIBODIES

Authors
TARASSISHIN LA RUSSELL WC
Citation
La. Tarassishin et Wc. Russell, A COMPETITIVE AVIDIN-BIOTIN ECL DOT-BLOT ASSAY FOR EPITOPE MAPPING OFMONOCLONAL-ANTIBODIES, Journal of virological methods, 49(1), 1994, pp. 89-92
Citations number
4
Categorie Soggetti
Virology,"Biochemical Research Methods
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
49
Issue
1
Year of publication
1994
Pages
89 - 92
Database
ISI
SICI code
0166-0934(1994)49:1<89:ACAEDA>2.0.ZU;2-4
Abstract
Using antigen-soaked nitrocellulose sheets stamped to Teraski plates, a microtechnique was devised for detecting bound biotinylated monoclon al antibodies which readily demonstrated non-overlapping epitopes. Thi s procedure is simple, sensitive and rapid and is useful in screening hybridoma supernatants.