THE EFFECTS OF 2'-ALKYL AND 3'-ALKYL SUBSTITUENTS ON OLIGONUCLEOTIDE HYBRIDIZATION AND STABILITY

Authors
SCHMIT C BEVIERRE MO DEMESMAEKER A ALTMANN KH
Citation
C. Schmit et al., THE EFFECTS OF 2'-ALKYL AND 3'-ALKYL SUBSTITUENTS ON OLIGONUCLEOTIDE HYBRIDIZATION AND STABILITY, Bioorganic & medicinal chemistry letters, 4(16), 1994, pp. 1969-1974
Citations number
37
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
16
Year of publication
1994
Pages
1969 - 1974
Database
ISI
SICI code
0960-894X(1994)4:16<1969:TEO2A3>2.0.ZU;2-8
Abstract
The hybridization properties and nuclease resistance of 2'- and 3'-alk yl, -heteroalkyl, -alkenyl, and -aryl substituted oligodeoxyribonucleo tides have been investigated. While such modified oligonucleotides gen erally exhibit reduced binding affinity for complementary RNA and DNA, a dramatic increase in stability against 3'-exonucleases was observed for certain 2'substituents.