A NEW CLASS OF 3'-MODIFIED NUCLEOTIDES AS CHAIN-TERMINATORS FOR DNA-SEQUENCING

Authors
FAULSTICH K HERRLEIN MK BRANDTNER S KONRAD RE GRIESINGER C ENGELS JW
Citation
K. Faulstich et al., A NEW CLASS OF 3'-MODIFIED NUCLEOTIDES AS CHAIN-TERMINATORS FOR DNA-SEQUENCING, Bioorganic & medicinal chemistry letters, 4(16), 1994, pp. 1975-1978
Citations number
9
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
16
Year of publication
1994
Pages
1975 - 1978
Database
ISI
SICI code
0960-894X(1994)4:16<1975:ANCO3N>2.0.ZU;2-5
Abstract
We describe the use of 3'-modified thymidine nucleotides as potent cha in terminators in Sanger's DNA sequencing reactions by application of T7 DNA polymerase and Mn2+-ions. These nucleotides contain fluorescein which is linked directly or by mono-, di- and tetraglycine units to t he 3'-position of a 3'-amino-2'-3'dideoxyribose.