LARGE-SCALE SCREENING OF YEAST ARTIFICIAL CHROMOSOME LIBRARIES USING PCR

The construction of physical maps of the human genome using sequence-t agged site content mapping requires that thousands of PCR amplificatio ns be performed On this scab, measures to reduce cost and to increase throughput become serious considerations. We describe relatively simpl e measures developed in our laboratory that increase the rate at which these reactions can be performed in a cost-effective manner These mea sures have been extensively tested in our laboratory and are readily a pplicable in other laboratories including those performing library scr eening on a move modest scale.