SCREENMAX(TM) PLASMID MINI-PREP - SUPER RAPID PLASMID DNA EXTRACTION METHOD

An ultra-quick method for plasmid DNA extraction using the ScreenMax(T M) Plasmid Mini-Prep Kit was optimized. Since the method has Sewer ste ps than current methods, the entire process takes only 12 min for two sets of plasmid DNA extractions. DNA quality was excellent for further analyses, including DNA sequencing. The kit's new medium, MMB, provid ed 5 times the viable cell count and 7 times the dry cell weight compa red with the conventional LB medium. The yield of plasmid DNA was 5 ti mes better than from LB medium. From 200 mu L of culture, the maximum yield was 7.5 mu g. The ScreenMax Plasmid Mini-Prep is nor only simple and economical but also safe because of its mild reagents. This new p rocedure is both reliable and reproducible.