H. Lucas et al., A FUCOSE-CONTAINING EPITOPE POTENTIALLY INVOLVED IN GAMETE INTERACTION ON THE HUMAN ZONA-PELLUCIDA, Human reproduction, 9(8), 1994, pp. 1532-1538
The oligosaccharide moiety of human, porcine and bovine zonae pellucid
ae was studied with lectins and monoclonal antibodies specific for tri
- or tetra-saccharidic epitopes containing at least one terminal alpha
-L-fucose. Animal eggs were collected from follicular aspirates, human
eggs were collected from in-vitro fertilization and embryo transfer p
rogrammes and pooled into six groups. By direct immunofluorescence, th
e lectins reactivity was detected for the animal or the human zonae po
ols in the same way. Reactivity of Aleuria aurantia lectin demonstrate
d the presence of alpha-L-fucose terminal residues in the zonae from t
he three species studied. By indirect immunofluorescence, the 2-25 ant
ibody reactivity was detected in every pool of human zonae whereas the
re was no evidence of any antibody reactivity on animal zonae. Using a
n anti-Lewis-b blood group antibody (2-25), we observed expression of
this antigen as an intrinsic component of the human zona pellucida, in
dependently of patients' Lewis red blood cell phenotypes. Antibody 2-2
5 inhibited the spermatozoa-zona binding in a hemizona assay, suggesti
ng that this fucose-containing antigen could be part of a sperm - zona
receptor.