A. Merzak et al., CELL-SURFACE GANGLIOSIDES ARE INVOLVED IN THE CONTROL OF HUMAN GLIOMACELL INVASION IN-VITRO, Neuroscience letters, 177(1-2), 1994, pp. 44-46
The involvement of cell surface gangliosides in glioma cell invasion i
n vitro was examined using the ganglioside-specific antibody A2B5 and
Matrigel-coated 8-mu m porosity polycarbonate filters. Invasion of six
cell lines derived from glial tumours of different histological grade
s was found to be markedly inhibited by A2B5 (50-96% inhibition) in a
dose-dependent manner. Furthermore, exogenous gangliosides were found
to prevent cell invasion when they were incubated with cells during th
e invasion assay. These results suggest that cell surface gangliosides
are involved in glioma cell invasion in vitro, probably because of th
eir adhesion-promoting action to basement membrane components.