Ch. Lochmuller et al., RETENTION BEHAVIOR OF POLY(L-TRYPTOPHAN)S AND POLY(D,L-TRYPTOPHAN)S IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY, Journal of liquid chromatography, 17(14-15), 1994, pp. 3179-3189
The reversed-phase liquid chromatographic retention behavior of poly(l
-tryptophan)s and poly(d,l-tryptophan)s) whose molecular weight ranged
from 5.4 kD to 37.25 kD is examined using a C-8 chemically bonded sta
tionary phase and binary mobile phases of tetrahydrofuran (THF)-water
and ternary mobile phases of THF-water-methanol. The retention ofpoly(
l-tryptophan)s is compared with that of poly(d,l-tryptophan)s. Linear-
Solvent-Strength (LSS) model is examined in describing the retention b
ehavior. The effect of adding a third solvent to the binary mobile pha
se is also discussed.