THE EFFECT OF ALLOPURINOL ON NA-PEROXIDATION IN ISCHEMIC AND REPERFUSED RABBIT KIDNEY(K+ATPASE RELATED LIPID)

1. Na+K+ATPase is a membrane bound enzyme whose activity is essential for maintenance of cell viability. Lipid peroxidation changes membrane fluidity and enzyme activity. 2. The purpose of this study was to inv estigate the effect of allopurinol (free radical scavenger) on Na+K+AT Pase activity in rabbit kidney cortex membrane. In this in vivo study we created ischemia and reperfusion in rabbit kidneys. 3. Enzyme activ ity were low in ischemic and reperfused kidneys, compared to the contr ols. In allopurinol treated ischemic and reperfused groups, the levels of Na+K+ATPase activity were high compared to the untreated group. 4. It has been concluded that allopurinol may protect this enzyme activi ty.