Ip. Nunes et al., EPITHELIAL EXPRESSION OF HLA CLASS-II ANTIGENS AND FC-GAMMA RECEPTORSIN PATIENTS WITH ADULT PERIODONTITIS, Journal of clinical periodontology, 21(8), 1994, pp. 526-532
The distribution of HLA class II (DR, DP, DQ) and Fc gamma R (I, II, I
II) was analyzed in the epithelia of patients with advanced marginal p
eriodontitis using cryostat sections incubated with monoclonal antibod
ies (MoAb) against the Langerhans cell (LC) (CD1a) and various subtype
s of HLA class II and Fc gamma R, and the indirect immunofluorescence
technique. In the oral gingival epithelium (OGE), LC were concentrated
subjacent to the connective tissue papillae, while in the pocket epit
helium (PE), they were most abundant at the gingival margin, HLA-DP, D
Q, and DR stained LC in both OGE and PE. HLA-DQ(+) LC were significant
ly fewer than DP+ and DR(+) LC. HLA-DR also stained keratinocytes (KC)
in the whole extension of both OGE and PE. HLA-DP was also observed o
n KC, but not HLA-DQ. Fc gamma R II stained both LC and focal areas of
KC. In PE FC gamma R II+ LC were concentrated near the bottom of the
pocket, while in the OGE, they were concentrated at the gingival margi
n. Fc gamma R III was present only on KC, especially in the basal and
suprabasal layer. The results indicate that the epithelial cells are a
ctively involved in the development and maintenance of the inflammatio
n of periodontal disease.