Ja. Brown et al., CHROMOSOMAL ANEUSOMIES DETECTED BY FLUORESCENT IN-SITU HYBRIDIZATION ANALYSIS IN CLINICALLY LOCALIZED PROSTATE CARCINOMA, The Journal of urology, 152(4), 1994, pp. 1157-1162
Fluorescent in situ hybridization using 12 chromosome enumeration prob
es (for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 17, 18, X and Y) was us
ed to evaluate fresh tumor touch preparations from 40 randomly selecte
d radical prostatectomy specimens. Of the tumors 16 (40%) contained ch
romosomal aneusomies. Chromosome 8 was aneusomic in 9 tumors (23%). Ga
in of chromosome 7 was observed in 8 tumors (20%). Chromosome 17 was a
neusomic in 4 cases, and chromosomes 10, 11, 12, 18 and Y were each an
eusomic twice. Loss of chromosome 9 was observed in 1 tumor. Chromosom
es 4, 6, and X were never aneusomic. The percentage of monosomy 17 nuc
lei was 2 to 4 times the amount noted with the other autosomes for tum
or and benign tissue. Computer analysis demonstrated that these signal
s contained twice the signal density and were significantly different
(p <0.0001) than the single diploid chromosome 17 signals. This result
is consistent with homologous pairing of chromosome 17 in benign and
neoplastic prostate tissue. Anomalies of chromosomes 8 and/or 7 were p
resent in 14 of the 16 cases (88%) aneusomic by fluorescent in situ hy
bridization. High grade tumors were more Likely to be aneuploid on flu
orescent in situ hybridization (p <0.001). Tumors with chromosome 8 an
eusomies were of higher stage (p <0.05). Fluorescent in situ hybridiza
tion is more sensitive than flow cytometry for the detection of aneuso
my/aneuploidy. The prognostic relevance of these findings will require
further investigation.