CHROMOSOMAL ANEUSOMIES DETECTED BY FLUORESCENT IN-SITU HYBRIDIZATION ANALYSIS IN CLINICALLY LOCALIZED PROSTATE CARCINOMA

Citation
Ja. Brown et al., CHROMOSOMAL ANEUSOMIES DETECTED BY FLUORESCENT IN-SITU HYBRIDIZATION ANALYSIS IN CLINICALLY LOCALIZED PROSTATE CARCINOMA, The Journal of urology, 152(4), 1994, pp. 1157-1162
Citations number
18
Categorie Soggetti
Urology & Nephrology
Journal title
ISSN journal
00225347
Volume
152
Issue
4
Year of publication
1994
Pages
1157 - 1162
Database
ISI
SICI code
0022-5347(1994)152:4<1157:CADBFI>2.0.ZU;2-H
Abstract
Fluorescent in situ hybridization using 12 chromosome enumeration prob es (for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 17, 18, X and Y) was us ed to evaluate fresh tumor touch preparations from 40 randomly selecte d radical prostatectomy specimens. Of the tumors 16 (40%) contained ch romosomal aneusomies. Chromosome 8 was aneusomic in 9 tumors (23%). Ga in of chromosome 7 was observed in 8 tumors (20%). Chromosome 17 was a neusomic in 4 cases, and chromosomes 10, 11, 12, 18 and Y were each an eusomic twice. Loss of chromosome 9 was observed in 1 tumor. Chromosom es 4, 6, and X were never aneusomic. The percentage of monosomy 17 nuc lei was 2 to 4 times the amount noted with the other autosomes for tum or and benign tissue. Computer analysis demonstrated that these signal s contained twice the signal density and were significantly different (p <0.0001) than the single diploid chromosome 17 signals. This result is consistent with homologous pairing of chromosome 17 in benign and neoplastic prostate tissue. Anomalies of chromosomes 8 and/or 7 were p resent in 14 of the 16 cases (88%) aneusomic by fluorescent in situ hy bridization. High grade tumors were more Likely to be aneuploid on flu orescent in situ hybridization (p <0.001). Tumors with chromosome 8 an eusomies were of higher stage (p <0.05). Fluorescent in situ hybridiza tion is more sensitive than flow cytometry for the detection of aneuso my/aneuploidy. The prognostic relevance of these findings will require further investigation.