4 EPITOPES ON TURNER NECROSIS FACTOR-ALPHA DEFINED BY MURINE ANTITUMOR NECROSIS FACTOR-ALPHA MONOCLONAL-ANTIBODIES

Authors
KARIM B BELIARD R HUART JJ BOUREL D
Citation
B. Karim et al., 4 EPITOPES ON TURNER NECROSIS FACTOR-ALPHA DEFINED BY MURINE ANTITUMOR NECROSIS FACTOR-ALPHA MONOCLONAL-ANTIBODIES, Immunology letters, 41(2-3), 1994, pp. 139-145
Citations number
36
Categorie Soggetti
Immunology
Journal title
Immunology letters → ACNP
ISSN journal
01652478
Volume
41
Issue
2-3
Year of publication
1994
Pages
139 - 145
Database
ISI
SICI code
0165-2478(1994)41:2-3<139:4EOTNF>2.0.ZU;2-R
Abstract
Eight murine anti-TNF, monoclonal antibodies (mAb) were produced after immunization of BALB/c mice with rhTNF(alpha). Six of these mAbs were able to neutralize cytotoxic activity of TNFalpha against L929 cells. Two other mAbs had no neutralizing effect. Epitope mapping studies we re performed by ELISA and by using a BIAcore system (Pharmacia). The d escribed mAbs were allowed to define 4 different epitopes on TNFalpha. Three of them were involved in the binding of TNFalpha with its recep tor (cytotoxic neutralization of TNFalpha). Another epitope was define d by non-neutralizing mAbs.