ANTIBIOTIC-ACTIVITY AND ABSOLUTE-CONFIGURATION OF 8S-HEPTADECA-2(Z),9(Z)-DIENE-4,6-DIYNE-1,8-DIOL FROM BUPLEURUM-SALICIFOLIUM

A polyacetylene has been isolated from Bupleurum salicifolium. Its str ucture and absolute configuration were determined to be 8S-heptadeca-2 (Z),9(Z)-diene-4,6-diyne-1,8-diol [1] by means of H-1- and C-13-nmr sp ectroscopic studies, including (H)-C-13 heteronuclear correlation (HMQ C) and long-range correlation spectra with inverse detection (HMBC). I ts absolute configuration was determined by application of the Horeau method. This compound exhibited significant antibiotic activity agains t the Gram-positive bacteria Staphylococcus aureus and Bacillus subtil is. Also isolated during this investigation were the known compounds: betulin, herniatin, 6,7,8-trimethoxycoumarin, p-hydroxyphenethyl alcoh ol, pluviatolide, guamaroline, bursehernin, guayadequiol, kaerophyllin , and matairesinol dimethyl ether.