IL-1-ALPHA ANTIPROLIFERATIVE AND DIFFERENTIATIVE EFFECTS ON DAUDI LYMPHOMA-CELLS - MULTIPARAMETRIC ANALYSIS

Interleukin I alpha (IL-1) is a potent agent that induces a wide range of biological effects. In this study we analysed its effects on cell cycle progression and differentiation of Daudi lymphoma cells. The par allel analysis in light microscopy and cytofluorimetry by means of ant i-BrdU monoclonal antibodies showed a reduced rate of proliferation (S phase) with a G1 arrest. These features were confirmed by the lower i ncorporation of [H-3]-thymidine supporting the decrease in the rate of DNA synthesis. In addition this cytokine was able to induce different iation after 24 his of treatment as assessed by the increased expressi on of Fc receptors (FcR) and morphological criteria. This multiparamet ric analysis gives evidence to the sensitivity to this cytokine of thi s peculiar cell line.