IDENTIFICATION OF A CONDITIONALLY ESSENTIAL HEAT-SHOCK PROTEIN IN ESCHERICHIA-COLI

Authors
PERUSKI LF NEIDHARDT FC
Citation
Lf. Peruski et Fc. Neidhardt, IDENTIFICATION OF A CONDITIONALLY ESSENTIAL HEAT-SHOCK PROTEIN IN ESCHERICHIA-COLI, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1207(2), 1994, pp. 165-172
Citations number
28
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1207
Issue
2
Year of publication
1994
Pages
165 - 172
Database
ISI
SICI code
0167-4838(1994)1207:2<165:IOACEH>2.0.ZU;2-6
Abstract
Protein D48.5 was recognized as a heat-inducible protein of Escherichi a coli during the screening of a group of random, temperature-inducibl e Mud-Lac fusion mutants. Physiological and genetic analysis demonstra ted that (i) the structural gene for this protein, designated htpI, is a member of the sigma(32)-dependent heat shock regulon, (ii) at 37 de grees C the synthesis of protein D48.5 is nearly constitutive, increas ing slightly with growth rate in media of different composition, and ( iii) this protein is essential for growth at high temperature.