SEQUENCES OF VP6 GENES OF HUMAN ROTAVIRUS STRAIN RV3 AND ITS VACCINE DERIVATIVE

The nucleotide sequence of the genomic segment encoding the major anti gen, VP6, of human rotavirus strain RV3 was determined for the virus i solated from faeces. This was compared with the sequence of the cognat e gene of tissue culture-adapted candidate vaccine virus that was deri ved from RV3 and had been passaged 30 times. A single silent nucleotid e difference was detected between the two genes and the deduced amino acid sequence for both showed the highest degree of identity (greater than or equal to 97.5%) with the VP6 proteins of rotavirus strains bel onging to the same subgroup. The amino acid residues that might affect VP6 subgroup epitope type from RV3 and other viruses of the same or d ifferent subgroups were compared. The commonality of particular amino acids among strains of different subgroups suggests that the presence of all subgroup-specific amino acids may be necessary for subgroup det ermination.