STRUCTURE AND TUMOR NECROSIS FACTOR-INDUCING ACTIVITY OF DISPERSED PARTICLES OF A LIPID-A ANALOG, GLA-60, AND PHOSPHATIDYLCHOLINE MIXTURE

A synthetic monosaccharide-analogue of lipid A, GLA-60, was co-dispers ed with egg PC in aqueous solution. The size distribution and structur e of the dispersed particles were investigated by the dynamic light sc attering (DLS) and transmission electron microscopic (TEM) methods. Th e particles were of liposomal structure with homogeneous size. The lea kage of CF out of the liposomes in rat plasma was suppressed by augmen ting GLA-60. Fluorescence anisotropy measurements showed that GLA-60 i n lipid bilayers led to a rigid structure of the membranes. The activi ty of tumor necrosis factor (TNF) induction in mice increased with the content of GLA-60 in sonicated liposomes. The results showed that the se liposomes were useful as GLA-60 formulations.