Palmitic acid is the major saturated fatty acid in soybean [Glycine ma
x (L.) Merr.] oil. Reducing levels of palmitic acid should enhance the
nutritive value of soybean oil for human consumption. The low palmiti
c acid strain C1726 (81 g kg(-1) 16:0), carrying the fap1 allele, was
crossed with two strains developed independently of C1726, N79-2077-12
and N90-2013, both with about 60 g kg(-1) 16:0. Progenies from these
crosses were evaluated to determine genetic relationships controlling
palmitic acid in these two groups of strains. Palmitic acid concentrat
ions in the oil of F-2 seeds, F-3 seeds on F-2 plants, and seeds from
F-3 families were determined by gas chromatography. Transgressive segr
egates averaging 44 and 96 g kg(-1) 16:0 were identified in the segreg
ating populations. Both F-2 plants and F-3 families were identified th
at were either homozygous or heterozygous for loci controlling palmiti
c acid. Chi-square analyses for a 1 homozygous P1 type/1 homozygous P2
type/1 homozygous recombinant type (high 16: 0)/1 homozygous recombin
ant type (low 16:0)/12 plants or families heterozygous at either locus
demonstrated that two loci controlling levels of 16:0 were segregatin
g in these populations. A correlation coefficient of 0.97 calculated b
etween palmitic acid values of homozgous F-2 plants and their F-3 fami
ly means demonstrated that the homozygous classes were true breeding f
or palmitic acid. The locus in C1726 was fap1; the other locus, in N79
-2077-12 and N90-2013, has not been designated. These populations prov
ide additional sources of low palmitic acid to enhance the nutritional
value of soybean oil.