IMMUNOAFFINITY COLUMN CHROMATOGRAPHY AGAINST FORSKOLIN USING AN ANTI-FORSKOLIN MONOCLONAL-ANTIBODY AND ITS APPLICATION

Authors
YANAGIHARA H SAKATA R MINAMI H TANAKA H SHOYAMA Y MURAKAMI H
Citation
H. Yanagihara et al., IMMUNOAFFINITY COLUMN CHROMATOGRAPHY AGAINST FORSKOLIN USING AN ANTI-FORSKOLIN MONOCLONAL-ANTIBODY AND ITS APPLICATION, Analytica chimica acta, 335(1-2), 1996, pp. 63-70
Citations number
18
Categorie Soggetti
Chemistry Analytical
Journal title
Analytica chimica acta → ACNP
ISSN journal
00032670
Volume
335
Issue
1-2
Year of publication
1996
Pages
63 - 70
Database
ISI
SICI code
0003-2670(1996)335:1-2<63:ICCAFU>2.0.ZU;2-3
Abstract
Immunoaffinity column chromatography using anti-forskolin monoclonal a ntibodies appears to be far superior to previously published separatio n methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 mu g ml(-1). Fo rskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.