PIG-KIDNEY (LLC-PK1) CELL-MEMBRANE FLUIDITY DURING EXPOSURE TO GENTAMICIN OR TOBRAMYCIN

Authors
KOHLHEPP SJ HOU L GILBERT DN
Citation
Sj. Kohlhepp et al., PIG-KIDNEY (LLC-PK1) CELL-MEMBRANE FLUIDITY DURING EXPOSURE TO GENTAMICIN OR TOBRAMYCIN, Antimicrobial agents and chemotherapy, 38(9), 1994, pp. 2169-2171
Citations number
24
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
38
Issue
9
Year of publication
1994
Pages
2169 - 2171
Database
ISI
SICI code
0066-4804(1994)38:9<2169:P(CFDE>2.0.ZU;2-U
Abstract
The surface membrane properties of LLC-PK1, cells grown,vith and witho ut various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-ni trobenz-2-oxa-1,3-diazol 4-yl)dipalmitoyl-L-alpha-phosphatidylethanola mine (NBD-PE) and the percentage of NBD-PE free to diffuse after photo bleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to pr eexposure levels and the diffusion coefficient decreased.