DETERMINATION OF PURINE-BASES BY CAPILLARY ZONE ELECTROPHORESIS WITH WALL-JET AMPEROMETRIC DETECTION

A new and sensitive method for the simultaneous determination of purin e bases using capillary zone electrophoresis with vertical wall-jet am perometric detection was developed The separation of adenine, guanine, hypoxanthine, xanthine and uric acid was performed in an uncoated sil ica capillary (50 cmx25 mu m i.d.). A bundle of carbon fibers was used to fabricate the working electrode and the assay was optimized with r espect to detection potential, pH and buffer concentration. The depend ence of peak heights for these purine bases on their concentrations fr om 1.0 fmol to 0.2 pmol was linear. Under optimized conditions, the pr oposed method has been applied to analyze salmon testes DNA and human plasma with satisfactory results.