UNIQUE LOCALIZATION OF FUCOSYL GM1 IN RABBIT SPINAL-CORD AND PERIPHERAL-NERVE - IMMUNOHISTOCHEMICAL STUDY USING MONOCLONAL ANTI-FUCOSYL GM1ANTIBODY CRD73-6

We generated a murine monoclonal antibody (CRD73-6) for ganglioside fu cosyl GM1 using a homogenate of rabbit dorsal root ganglia as an immun ogen. CRD73-6 immunostained a subset of neurons and the satellite cell s surrounding them in rabbit dorsal root ganglia, unmyelinated fibers in the dorsal root, and dorsal horn of the spinal cord. After the tiss ue sections had been treated with acetone, CRD73-6 also immunostained large neurons and the perineuronal area around them in the venteral ho rn, and axons and myelin of the small myelinated fibers in the dorsal root. Unique localization of fucosyl GM1 implicates its possible role in cell adhesion and recognition in the nervous system.