Y. Sone et al., ALTERATION OF BETA-D-GLUCAN FROM EDIBLE MUSHROOM AFTER INJECTION INTOMOUSE PERITONEAL-CAVITY, Journal of nutritional science and vitaminology, 40(4), 1994, pp. 335-341
Tritium-labeled antitumor beta-D-glucan derivative (T-labeled glucan)
was prepared from the branched beta-1,3-D-glucan of an edible mushroom
, Volvariella volvacea, by its periodate oxidation followed by reducti
on with NaBT4. Twenty-three hours after T-labeled glucan had been inje
cted into the mouse peritoneal cavity, about 3% of the total radioacti
vity injected was found in the mouse serum. In spite of the fact that
T-labeled glucan had no affinity to the anion-exchange column before i
njection, about 50% of the labeled beta-D-glucan in the serum thus obt
ained was adsorbed onto the column. The labeled beta-D-glucan fraction
eluted from the column by salt gradient showed antitumor activity in
vivo.