R. Bernard et al., IMMORTALIZATION OF DIFFERENT PRECURSORS OF GLIAL-CELLS WITH A TARGETED AND TEMPERATURE-SENSITIVE ONCOGENE, Experimental cell research, 214(1), 1994, pp. 373-380
Different types of glial precursor cell lines were obtained after stab
le transfection of brain cells with the pJC-SVLTtsA vector carrying th
e tsA58 simian virus 40 large T (SVLT) gene driven by the promoter of
a gliotropic strain of JC papovavirus. The immortalized cells were con
ditional for growth: they expressed the SVLT antigen and proliferated
at 34 degrees C, but their growth was either reduced or arrested when
they were shifted to 39 degrees C. The differentiation characteristics
of four representative lines were more extensively studied. The CR15
and CM8 lines displayed properties of bipotential glial progenitors: t
hey were able to express oligodendrocyte markers at both temperatures,
but could differentiate into astrocytes only at 39 degrees C. In cont
rast, the CR19 and CM3r lines corresponded to more committed oligodend
rocyte precursors: they expressed various oligodendroglial markers but
they could not synthesize the glial fibrillary acidic protein. More p
articularly, the CM3r mouse cells displayed a typical oligodendrocyte
progenitor morphology and expressed the proteolipid protein mRNA. (C)
1994 Academic Press, Inc.