NOVEL LABELING AGENTS FOR IMMUNOASSAY BY TIME-RESOLVED ELECTROGENERATED CHEMILUMINESCENCE

In order to develop more efficient labelling agents for the binding as says based on time-resolved electrogenerated chemiluminescence, thirte en derivatives of (1-hydroxybenzene-2,6-diyl)bis(methylene acid) were synthesized. The aim was to study the influence of different substitue nts on the intensity of electrogenerated chemiluminescence (ECL) of th eir terbium(III) chelates. For comparison, also photoluminescent inten sities of these chelates were recorded at the terbium emission line co rresponding to the transition D-5(4) --> F-7(5) (546 nm). The highest ECL intensities were recorded for the parent compound and its 4-methyl derivative, whereas the photoluminescent intensity was highest for th e 4-cyano and 4-phenyl derivatives. There was no statistically signifi cant correlation of the ECL intensity with the Hammett substituent con stants.