A. Lazou et al., EFFECT OF ANAEROBIOSIS AND ANHYDROBIOSIS ON THE EXTENT OF GLYCOLYTIC ENZYME BINDING IN ARTEMIA EMBRYOS, Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 164(4), 1994, pp. 306-311
The effect of anaerobiosis and anhydrobiosis on the extent of binding
of glycolytic enzymes to the particulate fraction of the cell was stud
ied in Artemia salina embryos. During control aerobic development, tre
halase, phosphofructokinase and pyruvate kinase showed an increase in
the percentage associated with the particulate fraction which is consi
stent with the carbohydrate-based metabolism of Artemia embryos. Howev
er, anaerobiosis resulted in decreased enzyme binding for six glycolyt
ic enzymes; hexokinase, aldolase, pyruvate kinase and lactate dehydrog
enase were the exceptions. Decreased enzyme binding was also observed
after exposure to dehydrating conditions. The results suggest that gly
colytic rate could be regulated by changes in the distribution of glyc
olytic enzymes between free and bound forms in Artemia embryos. This r
eversible interaction of glycolytic enzymes with structural proteins m
ay account for part of the metabolic arrest observed during anaerobic
dormancy and anhydrobiosis.