THE LEUKOCYTE RESPONSE OF JAPANESE-QUAIL TO ROUS-SARCOMA VIRUS-INDUCED TUMORS

Citation
Me. Janes et al., THE LEUKOCYTE RESPONSE OF JAPANESE-QUAIL TO ROUS-SARCOMA VIRUS-INDUCED TUMORS, Avian diseases, 38(3), 1994, pp. 610-615
Citations number
22
Categorie Soggetti
Veterinary Sciences
Journal title
ISSN journal
00052086
Volume
38
Issue
3
Year of publication
1994
Pages
610 - 615
Database
ISI
SICI code
0005-2086(1994)38:3<610:TLROJT>2.0.ZU;2-9
Abstract
In male Japanese quail, different circulating leukocyte responses were observed for progressors (birds developing a massive tumor that persi sted throughout the experiment) and regressors (birds developing a tum or that gradually disappeared) after initial challenge with Rous sarco ma virus (RSV). Blood was sampled before and at weekly intervals posti noculation. Blood smears were prepared and stained with Diff Quik, and a light microscope (1000x) was used in a direct count of 50 fields. L eukocytes were classified as heterophils, lymphocytes, monocytes, or e osinophils. The significant increase (P < 0.05) in total leukocytes at 14 days in regressors and progressors was consistent with the increas e in tumor growth. The regressors' individual percentage of leukocytes did not deviate from control values, whereas the progressors' percent ages of heterophils and monocytes were significantly higher (P < 0.05) and of lymphocytes significantly lower (P < 0.05) than those of contr ols by 14 days postinoculation. Indicative of this was the progressors ' heterophil to lymphocyte ratio, which was significantly higher (P < 0.05) than that of controls 14 days post RSV challenge and remained el evated throughout the experiment. These findings suggest that the prog ressors' immune response is suppressed by proliferation of malignant c ells. Therefore, the heterophil to lymphocyte ratio may be used in add ition to tumor size to identify those birds that will regress RSV-indu ced tumors.