Mild to marked splenomegaly was observed in three of four rheas that d
ied acutely in three unrelated commercial ratite facilities in Southea
stern Louisiana. Mortalities occurred within a 5-week period in birds
ranging from 2 months to 3 years of age. Multifocal hepatic and spleni
c necrosis with mononuclear cell infiltrates, typical of chlamydiosis
in other avian species, was present on histopathologic sections. The d
iagnoses were confirmed by demonstration of inclusion bodies in spleni
c impression smears and fluorescent-antibody testing performed on Vero
cell cultures.