RECOVERY OF LACTOPEROXIDASE FROM ACID WHE Y BY IMMOBILIZED COPPER AFFINITY-CHROMATOGRAPHY

Authors
DESTEFANO G PIZZUTO P SCIANCALEPORE V
Citation
G. Destefano et al., RECOVERY OF LACTOPEROXIDASE FROM ACID WHE Y BY IMMOBILIZED COPPER AFFINITY-CHROMATOGRAPHY, Industrie alimentari, 33(325), 1994, pp. 387-389
Citations number
NO
Categorie Soggetti
Food Science & Tenology
Journal title
Industrie alimentari → ACNP
ISSN journal
0019901X
Volume
33
Issue
325
Year of publication
1994
Pages
387 - 389
Database
ISI
SICI code
0019-901X(1994)33:325<387:ROLFAW>2.0.ZU;2-1
Abstract
Lactoperoxidase(E.C. 1.11.1.7.) from acid whey was recovered by immobi lized copper affinity chromatography using, as starting buffer, 0.05 M Tris-acetic acid, 0.5 M NaCl, pH 8. Eluition was performed stepwise w ith the same buffer by varying the pH from 8.0 to 2.5. Partially purif y bioactive enzyme was released at pH 4 with a recovery of 10 mg from 200 ML of acid whey.