CLONING OF METALLOPROTEASE GENES IN THE CARPET VIPER (ECHIS-PYRAMIDUM-LEAKEYI) - FURTHER MEMBERS OF THE METALLOPROTEASE DISINTEGRIN GENE FAMILY/

The genes coding for two metalloproteases, EcH-I and EcH-II, have been cloned from an Echis pyramidum leakeyi venom gland cDNA library. The cDNA sequences predict two zymogen molecules with strong amino acid se quence similarity and the same domain structure present in other membe rs of the viper metalloprotease/disintegrin gene family. EcH-I and EcH -II contain pro-protein, enzyme and disintegrin domains. Analysis of t he cDNAs coding for EcH-I, EcH-II, jararhagin, trigramin and Ht-e reve als a strong similarity, particularly in the untranslated regions and regions coding for the pro-peptide. Comparison of EcH-I and EcH-II wit h venom metalloproteases, mammalian matrix-degrading metalloproteases, sperm proteins, and a potential tumour suppressor gene highlights the presence of a number of motifs with potential functional significance .