IDENTIFICATION OF RECIPIENT RH PHENOTYPE IN A CHRONICALLY TRANSFUSED CHILD BY 2-COLOR IMMUNOFLUORESCENCE

A child with hyporegenerative anaemia was chronically transfused with group 0 Rh-negative blood. As typing with anti-D showed a mixed-field pattern, the patient's red blood cells (RBC) were identified as D posi tive. Due to the transfused ccddee RBC, it was impossible to determine by simple agglutination whether c was present on the patient's RBC. T o resolve this question, two-colour indirect immunofluorescence was pe rformed using D as a marker of the patient's RBC. Likewise, Duffy and Kidd antigens could be determined on the patient's RBC despite multipl e transfusions.