CRYSTALLIZATION OF A HYPERTHERMOPHILIC ARCHAEAL ELONGATION-FACTOR 1-ALPHA

Authors
ZAGARI A SICA F SCARANO G VITAGLIANO L BOCCHINI V
Citation
A. Zagari et al., CRYSTALLIZATION OF A HYPERTHERMOPHILIC ARCHAEAL ELONGATION-FACTOR 1-ALPHA, Journal of Molecular Biology, 242(2), 1994, pp. 175-177
Citations number
18
Categorie Soggetti
Biology
Journal title
Journal of Molecular Biology → ACNP
ISSN journal
00222836
Volume
242
Issue
2
Year of publication
1994
Pages
175 - 177
Database
ISI
SICI code
0022-2836(1994)242:2<175:COAHAE>2.0.ZU;2-N
Abstract
Intact and fully active elongation factor aEF-1 alpha from the hyperth ermophilic archaeon Sulfolobus solfataricus has been crystallized as a complex with GDP. Crystals were stable at temperatures below 8 degree s C and showed significant diffraction beyond 3.0 Angstrom. The orthor hombic lattice parameters were a = 62.9 Angstrom, b = 81.3 A, c = 115. 6 Angstrom with one molecule per asymmetric unit.