EPIDEMIOLOGIC MARKERS OF ACINETOBACTER-BAUMANNII CLINICAL ISOLATES FROM A SPINAL-CORD INJURY UNIT

Citation
Ma. Marcos et al., EPIDEMIOLOGIC MARKERS OF ACINETOBACTER-BAUMANNII CLINICAL ISOLATES FROM A SPINAL-CORD INJURY UNIT, The Journal of hospital infection, 28(1), 1994, pp. 39-48
Citations number
28
Categorie Soggetti
Infectious Diseases
ISSN journal
01956701
Volume
28
Issue
1
Year of publication
1994
Pages
39 - 48
Database
ISI
SICI code
0195-6701(1994)28:1<39:EMOACI>2.0.ZU;2-6
Abstract
During a period of 28 months, 114 isolates of Acinetobacter baumannii obtained from urine samples of 57 patients, were recovered in a Spinal Cord Unit; an unusual increase in the number of A. baumannii isolates was observed between February 1991 and January 1992. Six different ty ping methods [biotyping, antimicrobial susceptibility, whole cell and cell-envelope protein analysis, plasmid analysis and chromosomal DNA a nalysis by pulsed-field gel electrophoresis (PFGE)] were used to study the isolates to establish any potential relationships among them. Chr omosomal DNA analysis by digestion with ApaI and separation of the fra gments by PFGE was the most powerful tool to determine the relatedness of isolates. The results suggest that the isolates from 1991 and 1992 may have originated from strains present in 1990 that subsequently ac quired resistance to amikacin and tobramycin during the epidemic.