Ts. Skoglund et al., A METHOD FOR 2D RECONSTRUCTION OF INTRACELLULARLY LABELED NEURONS FROM SEQUENTIAL SECTIONS, Journal of neuroscience methods, 53(2), 1994, pp. 199-202
A technique for 2D reconstruction of intracellularly labeled neurons f
rom sequential sections is described. The system consists of a Charged
Coupled Device-camera mounted on a microscope, a videomixer and a IBM
-compatible PC with a framegrabber. The neurons (interneurons from the
spinal cord of the cat) were labeled iontophoretically by horshradish
peroxidase and subsequently cut in 60 mu m sections. The sections wer
e aligned using the video mixer by fitting the cut dendrites and axon
from one section with their counterparts in the following section. The
images were then digitized in the PC where they were fused to create
a superimposed picture of the aligned parts of the neuron; a 2D recons
truction was created.