MIXED ANTIGLOBULIN REACTION AND TRAY AGGLUTINATION-TEST FOR DETECTIONOF SPERM ANTIBODIES

Objective-To compare levels of sperm-agglutinating antibodies in male and female sera, cervical ovulatory mucus, and seminal plasma by two d ifferent tests. Patients and Methods-408 women and 404 men with long-l asting unexplained infertility and recurrent IVF failure were immunolo gically examined. Tray agglutination and mixed antiglobulin reaction t ests were used for the comparison of sperm-agglutinating antibodies in sera, in ovulatory cervical mucus, and in seminal plasma. Results-Hig h secretory IgA positivity predominated in seminal plasma in 16 (3.9%) of infertile men and in cervical ovulatory mucus in 114 (27.9%) of in fertile women. Positivity in two sperm antibodies together, in IgG and IgA, and IgM and IgE was also observed. Immunological- anti-sperm ant ibodies in sera indicate high levels of sperm antibodies in IgG and Ig M. Conclusion-Sperm immunoglobulin specification is important for ther apeutic influence upon immunocompetent cells.