CHEMILUMINOMETRIC DETERMINATION OF CHOLINE-RELATED SUBSTANCES IN PHARMACEUTICAL PREPARATIONS BY DOT-BLOT

A simple and reliable method of assaying succinylcholine chloride, oxt riphylline (choline theophyllinate) and acetylcholine chloride in phar maceutical preparations, based on conversion to choline, is provided b y combination of chemiluminometry of choline and dot-blot technique. R ecoveries of 102 and 97% for choline chloride in Quelicin injection an d Choledyl 200 tablets matrices, respectively, and of 99% for acetylch oline chloride in Miochol solution matrix could be achieved using simp le choline chloride standards in phosphate buffer pH 8.6. Accordingly, matrix matched standards were found redundant. Favourable results obt ained in preparations-matched media including limits of detection of 3 7-39 pmol mu l(-1) of choline chloride, together with accuracies of 0- 2% and RSDs ranging from 4 to 7% are the further evidence of the suita bility of the method.