HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF METAIODOBENZYLGUANIDINE IN WHOLE-BLOOD AND PLASMA OF CANCER-PATIENTS

An accurate and simple high-performance liquid chromatographic (HPLC) assay is presented for the quantitative determination of metaiodobenzy lguanidine (MIBG) in human whole blood and plasma. The sample pretreat ment involves a solid-phase extraction on Bakerbond SPE cyano columns. The HPLC system comprises a mu Bondapak C-18 column and ammonium phos phate (pH 4.0; 25 mM)-acetonitrile (80:20, v/v) as the mobile phase. D etection is performed by UV absorbance measurements at 254 nm. Linear regression with weighting factor 1/x(2) yielded the smallest sum of pe r cent relative concentration residuals over the concentration range o f the assay (0.1-10 mu g ml(-1)). MIBG levels, at the end of a 3-h inf usion, in whole blood and plasma of carcinoid patients were measured a nd compared with the results obtained with radiodetection after additi on of iodine-131-labelled MIBG.