A COMPARATIVE-STUDY OF SUPPORTS FOR THE SYNTHESIS OF OLIGONUCLEOTIDESWITHOUT USING AMMONIA

Authors
AVINO A GARCIA RG DIAZ A ALBERICIO F ERITJA R
Citation
A. Avino et al., A COMPARATIVE-STUDY OF SUPPORTS FOR THE SYNTHESIS OF OLIGONUCLEOTIDESWITHOUT USING AMMONIA, Nucleosides & nucleotides, 15(11-12), 1996, pp. 1871-1889
Citations number
26
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
15
Issue
11-12
Year of publication
1996
Pages
1871 - 1889
Database
ISI
SICI code
0732-8311(1996)15:11-12<1871:ACOSFT>2.0.ZU;2-#
Abstract
A comparative study of the cleavage efficiency of succinyl, phthaloyl, oxalyl, 2-(2-nitrophenyl)ethyl, 9-fluorenylmethyl, and 2-nitrobenzyl supports in 0.5M DBU solutions is described. A decrease in cleavage ef ficiency is observed when small oligonucleotides containing thymidine are linked to the supports. In these conditions oxalyl supports gave t he best yields followed by 2-(2-nitrophenyl)ethyl and 8-fluorenylmethy l supports.