PROTECTIVE EFFECT OF TETRAMETHYLPYRAZINE AGAINST DAMAGES OF AORTIC ENDOTHELIAL-CELLS ELICITED BY LOW-DENSITY LIPOPROTEINS

Effects of tetramethylpyrazine (TMP) on endothelial cells damaged by l ow-density lipoproteins (LDL) were investigated, When endothelial cell s were incubated with LDL (1.5 mg protein.ml(-1)) the level of malondi aldehyde (MDA) was increased and the activity of superoxide dismutase (SOD) was inhibited, and levels of cGMP and epoprostenol were decrease d. TMP at concentrations of both 20 and 150 mg.L(-1) nullified the inh ibition of SOD activity and the reduction of cGMP and epoprostenol con tent elicited by LDL. However, the elevation of MDA content induced by LDL was negated by TMP only at 150 mgL(-1). TMP also caused a reducti on in MDA content and an increase of epoprostenol level in normal endo thelial cells. This study suggests that TMP protects endothelial cells against damages elicited by LDL and that the protection of TMP might be due to reduction in lipid peroxidation through stimulation of produ ction and/or release of epoprostenol.