LIPASE AND ACIDIC PHOSPHATASE FROM THE PSYCHROTROPHIC BACTERIUM PSEUDOMONAS-FLUORESCENS - 2 ENZYMES WHOSE SYNTHESIS IS REGULATED BY THE GROWTH TEMPERATURE

In the psychrotrophic bacterium Pseudomonas fluorescens, the productio n of several enzymes that otherwise differ in their cell location, gro wth phase production and inducibility appeared to be similarly regulat ed by the growth temperature. In order to determine the level of this regulation, the expression of the apo and lip genes encoding two of th ese enzymes, the acidic phosphatase and lipase, respectively, was stud ied at different temperatures. Both genes were optimally expressed at 17.5 degrees C, i.e., at the optimal temperature of production of the enzymes; however, the low level of activity at the highest temperature could be due to an additional post-transcriptional control.