M. Nakamura et al., HIGH POTASSIUM AND CYCLIC-AMP ANALOG PROMOTE NEURONAL SURVIVAL OF BASAL FOREBRAIN CHOLINERGIC NEURONS IN CULTURE FROM POSTNATAL 2-WEEK-OLD RATS, Developmental brain research, 81(2), 1994, pp. 218-229
We found depolarization-dependent promotion of survival of cultured ba
sal forebrain cholinergic neurons from postnatal 2-week-old rats. Over
30 mM KCl (high K+) as well as nerve growth factor (NGF) induced cons
iderably high choline acetyltransferase (ChAT) activity and the increa
se was potentiated by the addition of BAY K8644, a Ca2+ channel agonis
t. The increase in ChAT activity by high K+ was due to the increased n
umber of viable acetylcholinesterase-positive and ChAT-positive neuron
s. Also, a cyclic AMP analog gave the same effect as high K+, but its
ability to induce the ChAT activity was higher than that of high K+. O
n the other hand, both high K+ and NGF had very little effects on the
survival of the cultured cholinergic neurons from 10-week-old rats. Cy
clic AMP analog induced considerable increase in ChAT activity and pro
motion of survival of cholinergic neurons in the 10-week-old culture.
These findings showed that the neuronal death occurring just of the en
d of synapse formation in rat basal forebrain cholinergic neurons coul
d be prevented by NGF and high K+, but the death of older cholinergic
neurons could not. We propose the possibility that the death of postna
tal 2-week-old basal forebrain cholinergic neurons in culture might be
programmed cell death.