OPTICAL SENSORS FOR BIOTECHNOLOGICAL APPLICATIONS

Different optical sensing systems based on fluorometric detection for biotechnological purposes are presented. A fibre fluorometric sensor i s described for monitoring the NADPH-dependent fluorescence of an enzy me complex from Z. mobilis for the analysis of glucose, fructose, gluc onolactone or sorbitol. Optical chemosensors for pH and oxygen concent ration were used as transducers for biooptodes for glucose, urea and p encillin. A two-channel device for the simultaneous monitoring of peni cillin and pH is presented. Two fibre optic based immunosensors are de scribed for the analysis of herbicides, mycotoxins and biotin. The use of the biotin sensor for the monitoring of a cultivation of recombina nt E. coli is discussed.